The methods of holographic endoscopy have recently emerged as a powerful platform to introduce sub-cellular resolution microscopy deep inside tissues of living organisms, including brain. Our laboratory of Complex Photonics (lead by Prof. Čižmár, http://www.isibrno.cz/cs/komplexni-fotonika) has been developing cutting edge multi-mode fibre based endoscopes and applying them in brain imaging in animal models in vivo. These minimally-invasive endoscopes can reach deep subcortical locations in the brain which are not accessible by other optical methods of similar resolution.
This PhD work will focus on expanding the capabilities of the system with two new modalities: optical manipulation of cells using optogenetics and extracellular electrophysiological recordings. Optogenetics is a unique tool that enables manipulation of cellular activity with light. Electrophysilogy has been a golden standard method of neuroscience for the past fifty years which allows recordings of local field potentials (LFP) and multi-unit activity (MUA) of neurons. Uniquely, these modalities will be implemented within the same thin endoscopic probe which serves for imaging. Implementation of electrophysiology will be carried out in collaboration with Prof. Massimo de Vittorio and Dr. Ferruccio Pisanello (Instituto Italiano de Technologia).
The candidate will develop/modify optical setups and the software for hardware control and data acquisition and processing (Lab View, Matlab, C++). Knowledge and experience in programing or optical set-up building is required. Experience with microscopy, fluorescence microscopy or electrophysiology is desirable.
The work will be carried out at the Institute of Scientific Instruments of the Academy of Sciences of the Czech Republic with the possibility of full-time employment. The PhD student will be a part of a European Commission project “DEEPER: Deep brain photonic tools for cell-type specific targeting of neural diseases" (https://www.deeperproject.eu/), which is funded within the European Union’s Horizon 2020 research and innovation actions scheme as is just starting at this institute. This Consortium clusters world-leading experts in molecular photonic tools, optical technology, pre-clinical, clinical brain research and innovative start-ups in an endeavour of providing new tools to tackle the mechanisms underlying the pathogenesis of neurological disease.
Supervisor: Dr. Hana Uhlířová
Discipline keywords: Applied Physics, Fiber optics, Endoscopy, Optical set-ups, In vivo imaging, Fluorescence, Optogenetics, Electrophysiology, Local field potentials, LFP, Multi-unit activity, MUA, MATLAB, Lab View, C++
The Center for Biomolecular Nanotechnologies at Istituto Italiano di Tecnologia - IIT in Arnesano, Lecce, Italy, is seeking for 3 (three) Postdocs interested in applying and developing new optical and optoelectronic devices for interfacing with the brain.
The successful candidates will join a thriving neuroscience and technology mix at the Center for Biomolecular Nanotechnologies of IIT, in a young and interdisciplinary research team composed by physicists, material scientists, engineers and neuroscientists. Our overall aim is to develop a new generation of neural interfaces based on novel photonic methods and conceptually new optoelectronic interactions with neural cells. Activities include basic research, market-oriented technology development and translational research of medical devices. The selected personnel will be expected to focus on the following challenging activities.
DEEPER is a research project funded from the European Union’s Horizon 2020 research and innovation actions (RIA) scheme under grant agreement no. 101016787.